pbluescript ii sk(+) cloning vector, ampicillin resistant  (Agilent technologies)

Journal:

Article Title: Contributions of MexAB-OprM and an EmrE Homolog to Intrinsic Resistance of Pseudomonas aeruginosa to Aminoglycosides and Dyes

doi: 10.1128/AAC.47.1.27-33.2003

Figure Lengend Snippet: P. aeruginosa strains and plasmids used in this study

Article Snippet: In the ethidium bromide accumulation experiments, M63 minimal medium [13.6 g of KH 2 PO 4 , 2 g of (NH 4 ) 2 SO 4 , and 0.5 mg of FeSO 4 per liter; pH 7.0] supplemented with glucose (0.2%) and MgSO 4 (10 mM) was used. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 P. aeruginosa strain or plasmid Description Source or reference P. aeruginosa PAO1 Prototroph 10 HN1112 Spontaneous streptomycin-resistant ( rpsL ) derivative of PAO1 This study HN1113 HN1112 Δ emrE This study K1119 PAO1 Δ mexAB-oprM 9 HN1114 Spontaneous streptomycin-resistant ( rpsL ) derivative of K1119 This study HN1115 HN1113 Δ mexAB-oprM This study K1589 Spontaneous streptomycin-resistant ( rpsL ) derivative of PAO1 made Δ mexR Δ mexB K. Poole HN1116 K1589 Δ emrE This study Plasmids pEX18Tc Broad-host-range gene replacement vector; sacB, tetracycline resistant 4 pXZL1307 pEX18Tc::Δ emrE This study pELCT04 pK18mob sacB ::ΩHg r and Δ mexAB-oprM, kanamycin and HgCl 2 resistant 9 pBluescript II SK(+) Cloning vector, ampicillin resistant Stratagene pXZL1582 pBluescript II SK(+):: emrE Pae This study Open in a separate window P. aeruginosa strains and plasmids used in this study Plasmids were maintained in E. coli with appropriate selection [pEX18Tc, 10 μg of tetracycline per ml; pECLT04, 50 μg of kanamycin per ml or 15 μg of HgCl 2 per ml; and pBluescript II SK(+), 100 μg of ampicillin per ml].

Techniques: Plasmid Preparation, Clone Assay